Biology Reference
In-Depth Information
CHAPTER
20
Top-Down Mass Spectrometry for
Protein Molecular Diagnostics and
B iomarker Discove ry
Steven M. Patrie, Michael J. Roth, Junmei Zhang
Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA
OUTLINE
Introduction
313
Orthogonal and Multidimensional Separations 322
Protein Heterogeneity
313
Informatics
323
Top-Down and Bottom-Up Proteomics
315
Current Status
324
Mass Spectrometry Hardware
for Top-Down
Top-Down Work
ows
324
315
Quantitation
324
Ionization
315
PTMs
325
Mass Analyzers and the Impact of Resolution
on Intact Protein Analysis
Membrane Proteins
325
318
Tissues and Bio
uids
326
Tandem Mass Spectrometry
319
Conclusion
326
Sample Preparation and Separations
320
Sample Preparation
321
References
326
High-Performance Liquid Chromatography
321
genetic code. A single translated gene may exist
in different isoforms resulting from genetic varia-
tion such asmutations and single-nucleotide poly-
morphisms (SNPs) that may also be differentiated
into a subset of protein species resulting from
alternative splicing (AS) and post-translational
INTRODUCTION
Protein Heterogeneity
The human proteome is diversi
edby chemical
and biological events occurring beyond the
 
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