Mass Spectrometry and NMR SpectroscopyeBased Quantitative Metabolomics - Proteomic and Metabolomic Approaches to Biomarker Discovery - page 280

(A)

Lipoproteins
(CH 2 ) n

Glycoproteins
( N -acetyl)

(CH 3 )

Albumin
(lysyl)

Lipoproteins
(= CH)

(B)

× 1.2

Ace

[2- 13 C]-Ace

Lac

BHB

Glc

β H2
Glc

AcA

α H1
Glc

Val
Leu
Ile

Crn

Ac

Cit

Ala

(C)

5.25 4.0 3.5 3.0 2.5 2.0 1.5 1.0

PPM

FIGURE 3 (A)

1 H NMR spectrum of blood plasma acquired with a low sensitivity toward diffusion (diffusion factor,

10,000 s/mm 2 , gray).
(B) 1 H NMR spectrum of blood plasma acquired with a high sensitivity toward diffusion (diffusion factor, b

4.1 s/mm 2 ), overlaid with a spectrum obtained with high diffusion sensitivity (diffusion factor, b

b ¼ ¼

10,000 s/mm 2 ).
The macromolecule spectra in (A) and (B) are identical. (C) Difference spectrum between (A) and (B). Abbreviations: acetate
(Ace), acetoacetate (AcA), aceton (Ac), alanine (Ala),

¼

-hydroxybutyrate (BHB), citrate (Cit), creatinine (Crn), glucose (Glc),
isoleucine (Ile), lactate (Lac), leucine (Leu), and valine (Val). (Reproduced with permission from reference #77.)

b