Biology Reference
In-Depth Information
CHAPTER
18
Mass Spectrometry and NMR
SpectroscopyeBased Quantitative
Metabolomics
Danijel Djukovic, G.A. Nagana Gowda, Daniel Raftery
Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University
of Washington, Seattle, WA, USA
OUTLINE
Metabolomics
279
Suppression of Macromolecular Signals
286
Quantitative Referencing
288
Comparative Chemometric Analysis versus
Quantitative Metabolomics
Spectral Simpli
cation Methods
289
280
Metabolite Quantitation Using
1D NMR
Mass Spectrometry
281
289
Liquid Chromatography Resolved MS
(LC-MS) Methods
Metabolite Quantitation Using 2D NMR
290
281
Isotope-Labeled NMR
291
Metabolite Quantitation Using LC-MS
282
Ex Vivo Isotope Labeling
291
Gas ChromatographyeResolved MS
(GC-MS) Methods
Conclusions
293
285
References
293
NMR Spectroscopy
285
Solvent Suppression
286
METABOLOMICS
metabolic responses of living systems to patho-
physiological stimuli or genetic alterations.
1,2
Although vast progress in the
fields of genomics
3
and proteomics
4
has provided a wealth of infor-
mation for understanding the factors that regu-
late cell physiology and pathology, great insight
Metabolomics (also known as metabonomics
ormetabolic pro
ling) dealswith the quantitative
analysis of multicomponent mixtures of biolog-
ical samples and is focused on establishing
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