Quantitative Proteomics in Development of Disease Protein Biomarkers - Proteomic and Metabolomic Approaches to Biomarker Discovery - page 252

CHAPTER
17

Quantitative Proteomics in Development
of Dis ease Protein Biom arkers

Xudong Yao, Adam J. McShane, Mary Joan Castillo
Department of Chemistry, University of Connecticut, Storrs, CT, USA

OUTLINE

Introduction 259 Multiple Reaction Monitoring or Selected

Reaction Monitoring MS

266

Quantitative Proteomic Pro ling for Protein

Technique Overview 266

Biomarker Discovery 260

Signature Peptides for Protein Targets 267

Common Techniques 261

Quantitation of Signature Peptides 268

Enzymatic 18 O-Labeling

261

High Resolution and High Mass Accuracy
MS

Chemical Tagging of Stable Isotope
Labels

269

261

Metabolic Labeling 263 Analyte Multiplexing and Sample

Throughput

Label-Free Quantitative Proteomics 264 270

Protein Biomarker Discovery 264

Conclusion 271

264

Differentially Expressed Proteins

265 Acknowledgments 272

Disease-Speci c Protein Isomers

Abnormal Protein Activities as Emerging
Biomarkers

References 272

265

Targeted Proteomic Validation of Biomarker
Candidates 266

quantitative proteomics in two major stages in
the development pipeline of protein biomarkers
for human diseases: the discovery of protein
biomarker candidates and the validation of the
protein candidates. 1

INTRODUCTION

This chapter discusses the technology and
application of mass spectrometry (MS) e based

The chapter concludes