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with lower
fidelity than enzymatic methods, and
although the alternative termini are all search-
able against proteomic databases, decreased
sensitivity results from multiple ions represent-
ing the same section of protein sequence. The
major advantages of Asp-selective microwave-
supported acid hydrolysis are speed and the
robustness of the proteolytic reaction.
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Analytical Chemistry
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International Journal of Mass Spec-
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e
11.
14. Hauser NJ, Basile F. Online microwave D-cleavage
LC-ESI-MS/MS of intact proteins: site-speci
301
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