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FIGURE 5 Product ion spectrum of the รพ 2 precursor ion at m/z 925.93 produced by Asp-selective acid hydrolysis and
tryptic digestion of a di-SUMO-2 conjugate. Note the b and y ion series identi
ed from both branches. (Modi
ed from reference
#17.)
using LC-MS/MS and MALDI-TOF MS. Rela-
tive abundances of the hemoglobin peptide
ions could be observed in a single spectrum,
correlating well with known expression levels
of each phenotype. 10
produce informative peptides in a fraction of
the time required for enzymatic proteolysis, and
its selectivity provides a robust alternative to
trypsin. The larger peptides produced by acid
hydrolysis offer several bene
ts: higher sequence
coverage of parent proteins, decreased sample
complexity via fewer peptides per protein, and
increased chromatographic separation ef
ADVANTAGES AND
DISADVANTAGES OF
MICROWAVE-SUPPORTED ASP-
SE LECTIVE ACID PROTEOLY SIS
ciency.
However, successful analysis of the higher
molecular weight species in ESI tandem mass
spectrometry requires high-resolution analysis
of both precursor and product ions in order to
assign charge states and masses, and accurate
mass measurements are achieved at the cost of
reduced throughput on some tandemmass spec-
trometers. Asp-selective acid hydrolysis occurs
Microwave-supported acid hydrolysis is
a rapid, kinetically selective method for effective
proteolysis of targeted proteins and complex
mixtures. This method has the capability to
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