Asp-Selective Microwave-Supported Acid Proteolysis - Proteomic and Metabolomic Approaches to Biomarker Discovery - page 218

CHAPTER
14

Asp-Selective Microwave-Supported
Acid Proteolysis

Joe R. Cannon, Catherine Fenselau
University of Maryland, College Park, Department of Chemistry and Biochemistry, College Park, MD, USA

OUTLINE

Introduction 225 Primary Sequence Studies of Human

Ribosomes

230

Aspartate-Selective Acid Proteolysis 226

Quantitation Using Isotope Ratios 231

Microwave-Supported Asp-Selective Acid
Proteolysis

Branched Proteins: SUMOylation 231

226

Advantages and Disadvantages of Microwave-
Supported Asp-Selective Acid Proteolysis Methods Development with Microwave-

Supported Acid Hydrolysis

233

227

References 235

Applications of Microwave-Supported Acid
Hydrolysis 230

INTRODUCTION

and colleagues 2 some years later to hypothesize
that hydrolysis proceeds via two mechanisms
in which the bond is cleaved between Asp and
either the adjacent N- or the adjacent C-
terminal residue. It has been shown that the reac-
tion can be catalyzed by different acids, as long
as the pH is maintained below the pK a of the
Asp side chain. Temperatures above 108 C are
also required for the reaction. In 2001, Li and
colleagues demonstrated that Asp-speci

Incubation in acid at high temperature has
long been employed to hydrolyze proteins in-
discriminately into their individual amino acid
constituents. In 1962, Schultz and colleagues
demonstrated that under acidic conditions that
were normally used for total protein hydrolysis,
aspartic acid cleavage occurred at a rate at least
100 times greater than any other amino acids. 1
Further characterization of the reaction led Inglis

c acid

cleavage was well suited for proteomics