Affinity Targeting Schemes for Biomarker Research - Proteomic and Metabolomic Approaches to Biomarker Discovery - page 212

FIGURE 8 A procedure for identification of carbonylated proteins and the sites of carbonylation. Panel A shows avidin
af nity chromatographic selection of biotinylated proteins from samples. Proteins thus selected were then resolved by RPC

(panel B). Following trypsin digestion, the peptide digest can either be identi ed directly by MALDI-MS (panel E) or subjected

to further fractionation by RPC (panel C). Peptide fractions collected from this RPC step are then identi ed by either