Affinity Targeting Schemes for Biomarker Research - Proteomic and Metabolomic Approaches to Biomarker Discovery - page 190

CHAPTER
13

Af nity Targeting Schemes for

B iomarker Researc h

Fred E. Regnier 1 , Wonryeon Cho 2
1 Department of Chemistry, Purdue University, West Lafayette, IN, USA,
2 Bio-Nano Chemistry, Wonkwang University, Iksan, Jonbuk, Korea

OUTLINE

Introduction 198 Glycan-Targeting Antibodies 210

The Unique Nature of Af nity Selection 198 Lectins 210

Prerequisites 199 Phosphorylation 214

Quanti cation 200 Antibodies 214

Immobilized Metal Af nity
Chromatography (IMAC)

Af nity Targeting Methods 202

214

Immune-Selection and Mass-Linked
Immuno-Selective Analysis (MALISA)

Titanium Oxide 216

202

Ion Exchange 216

Binding Proteins 205

Acetylation 216

Avidin/Streptavidin 205

Methylation 217

Aptamers 207

Oxidation 218

Immobilized Metal Af nity Chromatography

(IMAC) 207 Abundant Protein Removal 220

Single Gene Expression Product Selection 207 Conclusions 220

Post-Translational Modi cations 209 References 220

Glycosylation 209