Current Gel Electrophoresis Approaches to Low-Abundance Protein Marker Discovery - Proteomic and Metabolomic Approaches to Biomarker Discovery - page 169

CHAPTER
11

Current Gel Electrophoresis
Approaches to Low-Abundance
Prot ein Marker Disco very

Egisto Boschetti 1 , Giovanni Candiano 2 , Pier Giorgio Righetti 2
1 EB-Consult, Croissy, France, 2 Laboratory on Pathophysiology of Uremia,
G. Gaslini Children Hospital, Genova, Italy

OUTLINE

Introduction 175 Enrichment by the Reduction of Dynamic

Range 180

The Evolution of 2-DE Toward
Proteomics Applications 176 The Discovery of Protein Markers with

2-DE and Its Association with
Low-Abundance Protein Enrichment

Low-Abundance Proteins Are Not
Resolved by 2-DE Alone

181

178

Conclusions 187

Enhancing Low-Abundance Proteins 178

Proteome Fractionation: A Complex
Procedure with Protein Losses

Dedication 188

178

Acknowledgments 188

Depletion: A Biospeci c Method with Limited

Enrichment 179 References 188

not only for diagnosing pathological conditions
with precision but also for studying the impact
of novel molecules on protein expression, thus
contributing to drug discovery. Among specifi-

INTRODUCTION

Differential expression of proteins represents
one of the major

-

fields of investigation over the
last 15 years, with the promise of novel routes

cally developed technologies and tools in the eld

of electrophoretic separations, two-dimensional