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In-Depth Information
FIGURE 3 Comparison of the coverage in the ERK/MAPK pathway achieved by the MuDPIT and CITP proteomic
platform. Red: proteins only identi
ed by CITP; blue: proteins identi
ed by CITP with higher con
dence (larger numbers of
spectral counts and distinct peptide identi
cations per protein) than that achieved by MuDPIT; green: proteins identi
ed by
dence. 58
both CITP and MuDPIT with approximately equal con
responses, cell growth and survival, and drug
resistance to several chemotherapeutic agents. 61
remains, as cancer researchers explore the initial
steps in biological-signaling cascades and
compensatory processes. Besides sample com-
plexities, the greatest bioanalytical challenge
facing comprehensive proteomic and metabolo-
mic analysis of tumor specimens is related to
the identi
CONCLUSION
It has been well accepted that molecular
pro
cation of low-
abundance metabolites and proteins. Develop-
ments in capillary electrophoresis-based single
and multidimensional separations coupled with
MS detection and sequencing are particularly
highlighted for their roles within the broader
context of state-of-the-art clinical proteomic
cation and quanti
ling in tumor lesions is fundamental to
understand the molecular etiology in tumor
development and to provide the biomarkers
for early detection and prevention. Furthermore,
the need to detect small but biologically impor-
tant
changes
in metabolites
and proteins
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