Biology Reference
In-Depth Information
FIGURE 1
Base peak electrophero-
grams of human urine obtained from
(A) sheathless CZE-ESI-MS and (B)
CZE-ESI-MS using a sheath-liquid
interface.
24
individual separation dimension.
44
Due to the
proteome complexity of Mycobacterium marinum,
CZE-ESI-MS was therefore used as the second
separation dimension for further analysis of 11
tryptic digestion fractions generated from
RPLC.
45
CZE again favors the identi
In addition to human urine, CZE-ESI-MS was
also employed for the proteomic analysis of
other body
fluids such as human plasma
41
and
fluid.42
42
Potential
biomarkers of vascular disease in plasma from
patients with chronic kidney disease were
discovered.
41
In contrast to the application of
ESI-MS or ESI-MS/MS, of
ventricular
cerebrospinal
cation of
basic and hydrophilic peptides with low molec-
ular masses and is highly complementary to
RPLC toward the characterization of complex
proteome mixtures.
ine MALDI-TOF/
TOF-MS coupled with iTRAQ labeling
43
was
demonstrated for multiplexed quanti
cation of
proteins in human ventricular cerebrospinal
Capillary Isoelectric Focusing
CIEF/nano-RPLC separations coupled with
ESI-MS/MS have been developed and employed
to achieve comprehensive andultrasensitive anal-
ysis of minute protein digests extracted from
microdissected tissue specimens.
46,47
In addition
to protein identi
fluid samples collected from a patient with trau-
matic brain injury during patient recovery.
42
Capillary Electrophoresis-Based
Multidimensional Separations
Based on the high orthogonality of two sepa-
rations, the overall peak capacity is the multipli-
cation product of the peak capacity of each
cation, the use of label-free spec-
tral counting approach
48,49
dently and
reproducibly quantify protein expression levels
to con
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