Liquid Chromatographic Methods Combined with Mass Spectrometry in Metabolomics - Proteomic and Metabolomic Approaches to Biomarker Discovery - page 148

PC, SM, PE & DG

5.03

Positive ESI ion mode

LPC & LPE

2.25

5.60

5.47

4.95

TG & ChoE

3.66

4.78

8.82 9.00

5.93 6.06
6.18

1.46 1.71

8.60 9.22 9.31

9.47

4.64

1.96

1.11

0.59

Time

1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00

PG, PI, PS, PE

5.09

FA

Negative ESI ion mode

2.24

5.03

5.47 5.61
6.05

4.95

4.21 4.78

2.12

1.80

2.86

1.66

6.44
6.93

4.57 8.37

1.46

3.57

Time

1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00

FIGURE 3 Profiles from RP (upper) and HILIC (lower) -ToF-MS analysis of urine, obtained from Zucker lean and fat rats,
for the ion m/z160 showing the better retention of these polar substances on the HILIC phase . The PCA plots to the right of
the chromatograms show a clear separation (based on the whole metabolite pro les) between the two groups regardless of

which method of pro ling was used (but based on different markers). Red ¼ lean; black ¼ fat.