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RPLC - MZ 160
13W6
1: TOF MS ES+
160.604
1.79e3
5.36
100
100
80
60
40
9.01
20
0
-20
-40
2.30
-60
-80
2.43
-100
7.19
2.69
-100
100
0
4.99
3.87
4.79
5.75
0.09
0.73
SI MCA-P 11 - 16/ 11/ 2007 9: 04: 23 ì ì
0
Time
2.00
4.00
6.00
8.00
10.00
12.00
HILIC-MZ-160
13W6
1: TOF MS ES+
160.604
1.79e3
5.36
100
100
9.01
0
2.30
-100
2.43
7.19
2.69
4.99
3.87
4.79
5.75
-200
-100
0
100 200
SI MCA-P 11 - 16/ 11/
0.09
0.73
0
Time
2.00
4.00
6.00
8.00
10.00
12.00
FIGURE 2
Base peak chromatogram of human plasma extract. Retention time windows for the lipid classes are seen in the
UPLC-TOF MS chromatogram. Analysis was performed on a 1.8
2.1 mm i.d. Waters Acquity HSS T3
column at 55
C using gradient of acetonitrile:water (40:60, v/v) 10 mM ammonium acetate (eluent A) versus acetonitrile:
isopropanol (10:90, v/v) 10 mM ammonium acetate (eluent B). Elution started with 60% A; for 10 min the gradient was
ramped in a linear fashion to 100% B, where it was held for 2 min. The
m particle 100
m
flow rate was 0.4 mL/min and the injection volume
was 10
L. MS detection was performed on SYNAPT HDMS, in electrospray in both positive (top pane) and negative (bottom
pane) ionization. (Reprinted from reference 24, with permission from the American Chemical Society.)
m
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