Liquid Chromatographic Methods Combined with Mass Spectrometry in Metabolomics - Proteomic and Metabolomic Approaches to Biomarker Discovery - page 141

CHAPTER
9

Liquid Chromatographic Methods
Combined with Mass Spectrometry
in Metabolomics

Georgios A. Theodoridis 1 , Helen G. Gika 2 ,
Robert Plumb 3 , Ian D. Wilson 4
1 Department of Chemistry, Aristotle University, Thessaloniki, Greece,
2 Department of Chemical Engineering, Aristotle University, Thessaloniki, Greece,
3 Waters Corporation, Milford, MA, USA,
4 Biomolecular Medicine, Department of Surgery and Cancer, Faculty of Medicine,
Imperial College, London, UK

OUTLINE

Introduction 146 Detection 155

Chro matographic Methods for Metabolite
Pro

Quality Control, Data Analysis, and
Biomarker Detection

ling 147 156

Reversed-Phase LC Separations 147

Metabolite Identi cation and Biomarker

Hydrophilic Interaction Liquid
Chromatography (HILIC)

Validation 158

151

Other Approaches to the Pro ling of Polar Conclusions 159

and Ionic Metabolites 153

References 159

Miniaturized LC Systems 154