Using Data-Independent Mass Spectrometry to Extend Detectable Dynamic Range without Prior Fractionation - Proteomic and Metabolomic Approaches to Biomarker Discovery - page 115

CHAPTER
7

Using Data-Independent Mass
Spectrometry to Extend Detectable
Dynamic Range without Prior
Fractionation

Young Ah Goo 1 , 2 , David R. Goodlett 1
1 Department of Medicinal Chemistry, University of Washington, Seattle, WA, USA,
2 Department of Biobehavioral Nursing and Health Systems, University of Washington,
Seattle, WA, USA

OUTLINE

Introduction 120 Proteome Pro ling with PAcIFIC 127

Advancement in Mass Spectrometry 120 Human Plasma 127

Principle of the Precursor Acquisition
Independent from Ion Count (PAcIFIC)

Breast Cancer 127

121 Abdominal Aortic Aneurysm (AAA) 127

Recent Improvements to PAcIFIC 122

Perspectives 128

PAcIFIC and Quanti cation 123

References 129

Quantitative PAcIFIC (qPAcIFIC) 123

PAcIFIC with High-Resolution High Mass
Accuracy Precursor Ion Scans

124