Biology Reference
In-Depth Information
CHAPTER
1
Biomarker Discovery: Study Design
and Execution
Haleem J. Issaq, Timothy D. Veenstra
Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program,
Frederick National Laboratory for Cancer Research, Frederick, MD, USA
OUTLINE
Abbreviations
2
Ethnicity, Sex, and Age
8
Sample Collection, Handling, and Storage
8
Introduction
2
Method of Sample Analysis
9
De
nitions
2
Errors in Study Execution
9
Biomarker
2
Sample Preparation
9
Sensitivity
3
Methods of Analysis
10
Speci
city
3
Number of Replicates
10
Positive Predictive Value (PPV)
3
Effect of Mass Spectrometer Type
on the Results
Negative Predictive Value (NPV)
3
10
Proteomics
3
Effect of Separation Instrumentation
on the Results
Pro
ling
4
11
Metabolomics
4
Errors in Measurements
11
The Current State of Biomarker Discovery
4
Personnel and Experimental Validation
11
Study Design and Execution
5
Speci
city of Proteins as Biomarkers
12
Study Design
5
Published Results Comparison
12
Study Execution
6
Statistical Data Analysis
12
Personnel and Instrumentation
6
Recommendations
13
Errors in Study Design
6
The Sample
7
Concluding Remarks and Recommendations 13
Cancer Type and Stage
7
Acknowledgments
14
Sample Type
7
Selection of Patients and Controls
8
References
14
Number of Samples
8
Search WWH ::
Custom Search