Chemistry Reference
In-Depth Information
2
1 molar (M) copper (II) sulfate (CuSO
4
) solution
2
copper (II) sulfate solution of unknown concentration (between
0.1 and 1.0 M)
2
10-milliliter (ml) volumetric flask with stopper
2
graduated cylinder
2
test-tube rack
2
graph paper
2
goggles
2
science notebook
Safety Note
Goggles must be worn at all times during this
experiment. Use caution when using chemicals. please review and
follow the safety guidelines at the beginning of this volume.
Procedure
1. Turn on the spectrophotometer and allow it to warm up.
2. Label six cuvettes on the top rim with the following: 0 M, 0.25 M,
0.5 M, 0.75 M, 1.0 M, and unknown. Place the cuvettes in a test-
tube rack.
3. Your teacher will provide a 1.0 M solution of copper (II) sulfate and
distilled water (0.0 M). You will need to prepare the 0.25 M, 0.5 M,
and 0.75 M solutions of copper (II) sulfate by performing dilutions of
the 1.0 M solution. To do so:
a. Use the formula
M
1
V
1
=
M
2
V
2
, where
V
equals volume, to calculate
the volume of 1.0 M solution you will need to produce 10-ml
solutions with the desired concentrations (0.25 M, 0.5 M, and
0.75 M). Record your calculated values in the last column of
Data Table 1.
b. Measure out the calculated volume of 1.0 M CuSO
4
needed for
the 0.25 M dilution using a graduated cylinder.
c. Carefully pour the measured amount of solution into a 10 ml
volumetric flask and dilute up to the line with distilled water.
Place the stopper in the flask and invert several times to mix.
d. Pour the solution into a small labeled beaker and set aside for
use in step 4.
