Biology Reference
In-Depth Information
An additional layer of complexity lies in the number of
protein isoforms resulting from alternative splicing of
transcripts
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interactome maps available so far have mostly disregarded
isoforms, opting for a gene-centered approach for
simplicity and because differentiating between protein
isoforms is technically challenging. Isoforms of the same
protein may exhibit distinct combinations of protein
interaction interfaces, leading to distinct local interactome
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ACKNOWLEDGEMENTS
We thank past and current members of the Vidal Lab and the Center for
Cancer Systems Biology (CCSB) for their help and constructive
discussions over the course of developing our binary interaction
mapping strategies, framework, and protocols. We thank Dr David E.
Hill for insightful editing of this topic chapter and Dr Robin Lee for
providing an image of cells. This work was supported by National
Human Genome Research Institute grants R01-HG001715 awarded to
M.V. D.E.H, and F.P.R, P50-HG004233 awarded to M.V., R01-
HG006061 to M.V., D.E.H. and M.E.C., RC4-HG006066 to M.V and
D.E.H.; National Heart, Lung and Blood Institute grant U01-HL098166
(M.V. subcontract); National Institute of Environmental Health Sciences
R01-ES015728 toM.V.; National Cancer Institute grant R33-CA132073
to M.V.; National Science Foundation PGRP grant DBI-0703905 to
M.V. and D.E.H.; and by Institute Sponsored Research funds from the
Dana-Farber Cancer Institute Strategic Initiative awarded to M.V. and
CCSB. M.V. is a 'Chercheur Qualifi´ Honoraire' from the Fonds de la
Recherche Scientifique (FRS-FNRS, French Community of Belgium).
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