Biomedical Engineering Reference
In-Depth Information
Figure 2. Proposed pathway of biosynthesis of poly(γ-glutamic acid) from
B. subtilis
IFO 3335. (1)
Glutamate dehydronase; (2) Glutamate 2-oxoglutarate (α-ketoglutarate) aminotrasferase; (3) Glutamine
synthetase; (4) Pyruvic acid aminotransferase (L-Glutamic acid); (5) Alanine racemase; (6) Pyruvic
acid aminotransferase (D-Glutamic acid); (7) PGA synthetase (Source: Kunioka 1997).
Table 1. Synthesis of
γ
-PGA from various Bacillus strains
Cultivation
conditions
Yield
(g/L)
Molecular
weight
D : L
ratio
Refere-
nces
Bacillus
strains
Nutrients
1.4 x 10
4
-
9.8 x 10
5
B. licheniformis
ATCC 9945
Glutamic acid,
Glycerol,
17-23
45-85 :
55-15
[8]
37°C, 4
days
1.4 x 10
4
-
9.8 x 10
5
B. licheniformis
ATCC 9945
Citric acid,
NH
4
Cl,
17-23
45-85 :
55-15
[36]
37°C, 4
days
