Biomedical Engineering Reference
In-Depth Information
55 .2
10 0
67.2 7 9 .2
80
81 .2
60
40
167.0
91.2 95 .2
15 7 . 1
14 9 . 2
20
279.1
12 1. 2
19 1. 3
17 7 . 2
10 7 . 2
19 9 . 2
235.1
257.2
12 3 . 2
2 8 4.5
391.1
29 3.0
332.5
365.8
376 .9
419.7
429.9
502.5
44 1.0
462.6
481.7
0
50
10 0
15 0
200
250
300
350
400
450
500
m/z
(a)
67.2
10 0
80
60
79.2
107.2
81.2
40
12 1. 2
123 .2
13 7 . 1
20
149 .2
187.2
279.2
255.1
19 1. 2
24 1.2
227. 2
29 9.1
479. 4
493.4
301.1
333.2
353 . 2
369.2
391.1
411.4
4 2 3.2 443.1
461 .2
0
50
10 0
15 0
200
250
300
350
400
450
500
m/z
(b)
Figure 7. Mass spectrum of material eluted of RP-HPLC corresponded to the enzymatic synthesis
catalyzed by Acacia caven CE in a 50 % (v/v) ethyl acetate, using: a) (4mM) Z-Gln and (4mM)
Phe.OMe, after 30 min of reaction (Z-Gln-Phe.OMe ESMS [ M + 1] 441.0; Z-Gln ESMS [ M + 1] 279.1;
Phe.OMe ESMS [ M + 1] 177.2); b) (4mM) Z-Phe and (4mM) Phe.OMe, after 1h of reaction (Z-Phe-
Phe.OMe ESMS [ M + 1] 461.2; Z-Phe ESMS [ M + 1] 299.1; Phe.OMe ESMS [ M + 1] 177.2).
Conclusion
Enormous efforts over the past two decades to find detergent proteases and to improve
known ones have not succeeded in bringing forth a single new enzyme as versatile and
universally applicable as the high-alkaline subtilisin. At this moment, the investigation is
focused in finding new suitable enzymes to be used in specific environments such as low-
temperature automatic washing detergents, essentially due to the necessity to save energy and
to adapt to new fragile materials in clothing. Proteolytic extract of Acacia caven pollen
fulfilled all the requirements for its application in those detergent formulations: high stability
in a broad range of temperature (25-50° C), high activity in alkaline pH (7.5-10) and very
good compatibility with the commercial detergent additives.
By last, but not least important, this work reports a new plant protease with potential
application to the peptide synthesis and the laundry detergent industry, from a renewable
natural resource (an autochthonous plant) of the Latin American region. In this sense, we
believe that this research has novelty and in addition, the crude extract obtaining is very
simple and cheap, and it avoids the use of fermentation and genetic engineering.
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