Biomedical Engineering Reference
In-Depth Information
3.2
3.0
2.8
2.6
2.4
2.2
2.0
1.8
6
7
8
9
10
11
pH
Figure 1. Effect of pH on the proteolytic activity of
Acacia caven
CE, after 5 min at 37° C.
According to Figure 2a, temperature profile of
Acacia caven
CE showed a sharp peak of
maximal proteolytic activity at 35-40° C but high activities were obtained between 25 and 50°
C. Besides, the
Acacia caven
CE displayed a remarkable thermal stability after 1.5 h at 25-40°
C retaining 100 % of its initial activity, but it decreased as long as temperature increased to 60°
C (Figure 2b). Although the enzyme was very stable below 40° C after 1.5 h, only 25 % of the
initial proteolytic activity was retained at 60° C under similar incubation conditions. The high
thermostability of
Acacia caven
proteases below 40° C is desirable for laundry purposes and
from the ecological and economical point of view, mainly, because saving of energy.
3.5
a)
3.0
2.5
2.0
1.5
1.0
0.5
0.0
20
30
40
50
60
70
80
Temperature (ºC)
Figure 2. Continued on next page.
