Biomedical Engineering Reference
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Figure 9. Effect of methanol content on methanolysis of soybean oils. The conversion was expressed as
the amount of methanol consumed for the ester conversion of the oil (when the molar ratio of
methanol/oil was less than 3), and as the ratio of methyl ester to the oil (more 3) [32].
Figure 10. Time courses of ME content in a repeated methanolysis operation using immobilized-cells
with and without glutaraldehyde treatment [32].
can only be achieved with organic solvents. There is also a high cost of production of
enzymes and stringent controls which increases overall costs [27]. Enzymes can also be
immobilized into a substrate, though would require replacement once yields decrease [19].
Lin et al (2005) demonstrated that Rhizopus arrhizus was employed as whole-cell catalyst and
it can be immobilized in porous biomass support particles (BSPs) readily (see Figure 8). As
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