Biomedical Engineering Reference
In-Depth Information
growth yield under uncoupler addition conditions; K s/x is saturation constant related to S 0 /X 0 ;
K u/x is saturation constant related to C u /X; X is biomass concentration at the instant of
uncoupler addition; S 0 is initial substrate concentration.
3.5. Molecular Analyses
Microbial populations at the presence of uncouplers can be monitored by several
molecular analyses, such as PCR amplification of 16S rRNA genes followed by denaturing
gradient gel electrophoresis (DGGE). These two microbiological techniques are always
combined together to determine the molecular composition of the microbial community.
Besides, Polyacrylamide Gel Electrphoresis (PAGE) is also frequently used to analyze the
active biomass with small molecular weight RNA [87].
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