Biology Reference
In-Depth Information
FIGURE 16.7
Example of a modular clean room laboratory. Note that the laboratory has its own air filtration
system at the top of the unit; the inside of the room is positive pressured. All air, therefore, only comes in through
the ceiling filtration units and only goes out one door (seen to the very left). The room's inside is equipped with UV
lights that are turned on to “decontaminate” the room when not in use. Photo courtesy of Dr. Anne C. Stone,
Arizona State University.
predictions will help guide you towards the appropriate data source: Do you need biparen-
tally inherited nuclear DNA, or uniparentally inherited mtDNA and/or NRY? What level of
resolution do you need? That is, do you need broad evolutionary lineages, or do you want to
be able to be as individually identifying as possible? The answers to these questions will help
you decide what regions of the genome and kind of
genetic marker(s)
you should study and
how many markers you will need to best address your hypotheses.
Before Considering a Project Using Degraded DNA
.
make sure you have access to two very important things: (1) someone with extensive
experience working with degraded DNA and (2) a clean room laboratory. These two factors
are very important because while “modern DNA” techniques (i.e., DNA techniques oriented
towards high-quality DNA) appear the same as those applied to degraded DNA, they differ
extensively in the details. The following are just a very few examples of the differences
between working with modern versus degraded DNA: instead of standard chemical reagents
(that are sold with a certain degree of purity and sterility), you must order DNA-free
.
