Biology Reference
In-Depth Information
Target
Sequence
Genomic DNA
Denaturation:
Heat briefly to
separate DNA
strands
1.
Annealing:
Cool to allow
primers to bond
with ends of
target sequence
2.
Cycle 1 yields
2 DNA
sequences
(exact copies)
primers
3.
Extension of
primers: DNA
polymerase adds
new nucleotides
(nts) to end of each
primer
new
nts
Cycle 2 yields
4 DNA
sequences
(exact copies)
Cycle 3 yields
8 DNA
sequences
(exact copies)
and so on....
FIGURE 16.5
Polymerase chain reaction (PCR) steps.
