Bone and Dental Histology - Research Methods in Human Skeletal Biology - page 370

BOX 13.1 ( cont'd)

17 Using forceps, gently place the bone

section onto a thin layer of superglue
and adhere to slide ( Figure 13.12 ).

19 Polish the sample prior to examination

of histomorphological features under
the microscope. Polishing will remove
or lessen any scratches on the slide
from the grinding process.

18 If necessary, grind samples down using

grit paper. The slide can be placed in
a grinding fixture to secure it during
grinding ( Figure 13.13 ). The ideal thin
section is translucent against the light.
An example of a grinder/polisher that
can be used for slide preparation is
a Dual Wheel Mark V 8 Laboratory
grinder/polisher.

20 Save the remaining portions of the

blocks of embedded bone samples for
future research ( Figure 13.14 ).

6 Buehler, Lake Bluff, Illinois.
7 Leica Microsystems, Wetzlar, Germany.
8 Mark V Laboratory, East Granby, Connecticut.

FIGURE 13.9 Bone samples in peel-
away molds with embedding medium.