Photocrosslinkable Materials for the Fabrication of Tissue-Engineered Constructs by Stereolithography - Tissue Engineering: Computer Modeling, Biofabrication and Cell Behavior - page 163

Fig. 14 Gelatin-based scaffolds built by 2PP process. Bar corresponds to 1 mm [ 79 ]

Fig. 15 ( A ) Fabrication procedure used to create patterns of acryl-fibronectin on the backbone of
PEGDA hydrogels produced by stereolithography. ( B ) Fibroblasts aligned on the unpatterned and
patterned hydrogels substrates [ 23 ]

merization. Scaffolds were crosslinked with the benzophenone dimer, and struc-
tures at sub-micrometer and micrometer scale were obtained. Results showed that
the degradation rate of these structures can be tailored by changing the degree of
cross-linking of collagen during fabrication. Primary human dermal fibroblasts cul-
tured on the collagen structures showed good adhesion, so there is biocompatibility
in the designed constructs. Chan et al. [ 23 ] developed a simple method for align-
ing cells on 3D hydrogels, by combining the micro-contact printing technique and
stereolithography (Fig. 15 ). They modified fibronectin with acrylate groups, creat-
ing patterns of acryl-fibronectin on the backbone of PEGDA hydrogels produced by
stereolithography, using a micro-contact printing technique. The cell alignment in
the direction of the patterns created on the hydrogel substrates, was showed by the
culture of the NIH/3T3 mouse embryonic fibroblasts.