Rational Design of Artificial Cellular Niches for Tissue Engineering - Tissue Engineering: Computer Modeling, Biofabrication and Cell Behavior - page 140

5 Concluding Remarks
We have emphasized the importance of a rational design of in vitro models to ac-
celerate advances in Tissue Engineering. Such advances might benefit the coordi-
nated efforts spent in different disciplines including (i) in silico modeling; (ii) fab-
rication of smart 3D systems (taking advantage of innovative biomimetic materials
and/or biofabrication techniques); and (iii) development of miniaturized (microflu-
idic) controlled environments for in vitro experiments.
It is an opinion of the Authors that the most significant contributions in the field
of Tissue Engineering are to be expected from multidisciplinary approaches merging
competences across these domains.
References
1. Frantz C, Stewart KM, Weaver VM (2010) The extracellular matrix at a glance. J Cell Sci
123:4195-4200. doi: 10.1242/jcs.023820
2. Oh J, Recknor JB, Recknor JC, Mallapragada SK, Sakaguchi DS (2009) Soluble factors from
neocortical astrocytes enhance neuronal differentiation of neural progenitor cells from adult
rat hippocampus on micropatterned polymer substrates. J Biomed Mater Res, Part A 91:575-
585. doi: 10.1002/jbm.a.32242
3. Nelson CM, Vanduijn MM, Inman JL, Fletcher DA, Bissell MJ (2006) Tissue geometry deter-
mines sites of mammary branching morphogenesis in organotypic cultures. Science 314:298-
300. doi: 10.1126/science.1131000
4. Mori H, Gjorevski N, Inman JL, Bissell MJ, Nelson CM (2009) Self-organization of engi-
neered epithelial tubules by differential cellular motility. Proc Natl Acad Sci USA 106:14890-
14895. doi: 10.1073/pnas.0901269106
5. Discher DE, Janmey P, Wang YL (2005) Tissue cells feel and respond to the stiffness of their
substrate. Science 310:1139-1143. doi: 10.1126/science.1116995
6. Sarkar P, Rao BM (2009) Molecular aspects of cardiac differentiation in embryonic stem cells.
Crit Rev Biomed Eng 37:283-320. doi: 00f1eff12a65576b
7. Leong WS, Tay CY, Yu H, Li A, Wu SC, Duc DH, Lim CT, Tan LP (2010) Thickness sensing
of hMSCs on collagen gel directs stem cell fate. Biochem Biophys Res Commun 401:287-
292. doi: 10.1016/j.bbrc.2010.09.052
8. Reilly GC, Engler AJ (2010) Intrinsic extracellular matrix properties regulate stem cell differ-
entiation. J Biomech 43:55-62. doi: 10.1016/j.jbiomech.2009.09.009
9. Gang Z, Qi Q, Jing C, Wang C (2009) Measuring microenvironment mechanical stress of
rat liver during diethylnitrosamine induced hepatocarcinogenesis by atomic force microscope.
Microsc Res Tech 72:672-678. doi: 10.1002/jemt.20716
10. Dulinska I, Targosz M, Strojny W, Lekka M, Czuba P, Balwierz W, Szymonski M (2006) Stiff-
ness of normal and pathological erythrocytes studied by means of atomic force microscopy.
J Biochem Biophys Methods 66:1-11. doi: 10.1016/j.jbbm.2005.11.003
11. Klein TJ, Malda J, Sah RL, Hutmacher DW (2009) Tissue engineering of articular cartilage
with biomimetic zones. Tissue Eng, Part B Rev 15:143-157. doi: 10.1089/ten.TEB.2008.0563
12. Vinatier C, Guicheux J, Daculsi G, Layrolle P, Weiss P (2006) Cartilage and bone tissue
engineering using hydrogels. Biomed Mater Eng 16:S107-S113
13. Balakumaran A, Robey PG, Fedarko N, Landgren O (2010) Bone marrow microenvironment
in myelomagenesis: its potential role in early diagnosis. Expert Rev Mol Diagn 10:465-480.
doi: 10.1586/erm.10.31