Structural Characteristics Changes in Erythrocyte Membranes of Mice Bearing Alzheimer’s-like Disease Caused by the Olfactory Bulbectomy - Progress in Organic and Physical Chemistry: Structures and Mechanisms - page 51

The MDA content and hemolysis were measured before (mechanical hemolysis)
and after (peroxile hemolysis, PH) incubation at 37°C by the reaction with thiobarbi-
turic acid.

5.3 DISCUSSION AND RESULTS
Spin correlation time alterations of the spin probes in the erythrocytes membrane of
the BE and ShO mice are shown in Figure 1(a). Inverse relation between probe I and
probe II spin correlation time changes were found in the erythrocyte membranes of
ShO mice for the first 4 months after operation. Usually, such antibate relation is
observed in health [5]. After 4 months there are symbatic alterations in probe I and
probe II spin correlation time. Apparently lipid composition is changed due to aging
processes in this case. It is worthy of note that microviscocities of both region, lipidic
and near-protein, of the erythrocyte, and the synaptosomal membranes, shown at [1],
are changing in opposite way for ShO mice.
One can see more complicated alterations for BE mice (Figure 1(b)). Relative
membranes microviscosity changes are shown in Figure 2 (ShO mice considered as
control group).

FIGURE 2 The BE mice erythrocyte membrane relative microviscocity alterations.

Microviscosity of the erythrocyte as well as forebrain [1] membranes lipid
phase were deeply decreased at the early stage of the dementia development. En-
hanced À uidity of the lipid phase leads to increase the liability of the near-protein
region of erythrocyte membranes. At the 3rd month after olfactory bulbectomy
microviscosity of the BE mice erythrocyte membranes returned to normal value.
Thereafter À uidity of the both lipidic and near-protein membranes region was
symbatic growing.