Chemistry Reference
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2,306 metaphases with rearrangements (13.17%). Alterations of chromosomal type
made less than 1-0.113%. This magnitude is similar to the natural frequency. At a
concentration of phosphemid of 2 × 10
-3
M mitotic activity was on the average 51.5%
(Table 2), that is two times lower than in the controls (94.9%) (Table 1).
TABLE 2
Aberrations of chromosomes in 2n-meristem cells of
Crepis capillaris
after 24 and
27 hr from the start of treatment of seeds in a solution of phosphemid 2 × 10
-3
M (2. 24 mg, 50
ml of water). Fixation through 3-8 hr after arising. Seeds were germinating in 0.01% solution
of colchicine. Harvest of 1967 year. The analysis in April 1968.
Time, in hours
Number of investigating
seedlings
Metaphases
Rearrange-
ments of chro-
mosomal type
from soaking
up to «aris-
ing»
from soaking
up to fixation
Ȉ with metaphases Ȉ with rearrange-
ments, %±
Ȉ
%
24
3
57
24
42.1
741
9.4 ± 1.08
0
6
56
32
57.1
1270
17.2 ± 1.06
1
8
54
36
66.7
1815
14.0 ± 0.82
0
27
3
47
16
34.0
373
20.6 ±2.10
1
5
19
12
63.2
313
15.3 ± 2.04
0
Total
233
120
51.5 ± 3.28
4512
14.8 ± 0.53
0.043 ± 0.031%
Data of Table 2 show that at the weak concentration of preparation 2 × 10
3
with the
increase of term of ¿ xation in every “arising” mitotic activity increases, and frequency
of alterations diminish.
At max concentration 1 × 10
-2
M (Table 3), we registered the large number of
rearrangements nearly 60% through 27 hr after arising in term 24 hr, of which 16.4%
were mitosis with multiple rearrangements (it is marked an asterisk). On average,
signi¿ cant increase the number of strongly damaged metaphases was 13.3 ± 2.9%.
Rearrangements of chromosomal type at this dilution also did not ¿ nd.
Average frequency of mitotic activity of phosphemid in a concentration of
1 × 10
-2
M at the same quantity of seeds, as in Table 2, was lower almost twice 25%
(Table 3). Average number of metaphases with rearrangements increased nearly 22
versus
14.8%.
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