Chemistry Reference
In-Depth Information
19
On the Influence of
Melafen - Plant Growth
Regulator
O. M. Alekseeva and G. E. Zaikov
CONTENTS
19.1 Introduction ..................................................................................................... 241
19.2 Experimental Part ............................................................................................ 245
19.2.1 Materials .............................................................................................. 245
19.2.2 Methods ............................................................................................... 245
19.3 Discussion and Results ................................................................................... 246
19.4 Conclusion ....................................................................................................... 250
Keywords ................................................................................................................. 251
References ................................................................................................................ 251
19.1 INTRODUCTION
The effects of plant growth regulator Melafen to the some animal cell metabolic path-
ways were investigated at our work. Earlier literature data recognized that the fluctua-
tions of animal cellular volume are correlated with some Ca
2+
dependent cellular meta-
bolic pathways. The cellular volume was registered by spectral method - the first light
scattering, of diluted cellular suspensions that were incubated under the Melafen aqua
solutions at wide concentration region (10
-21
-10
-3
M). The Ehrlich ascetic carcinoma
(EAC) cells, that have the metabotropic purinoreceptors P2Y at its surface, showed
two maximums at light scattering kinetic curve that correlated with two maximums
of volumes increasing after the one addition of adenosine triphosphate (ATP). The
leukocytes, which have the Ca
2+
channel former purinoreceptors P2X at its surface,
showed that the lag phase at light scattering kinetic curve before the cellular volume
increasing after the ATP addition was in dependence of Melafen concentration. The
thymocytes, which have the Ca
2+
channel former purinoreceptors P2Z (and other too)
at its surface, showed two maximums at light scattering kinetic curve after the one
addition of ATP also. But the second answer that correlated with the CRAC activa-
tion was in bimodal dependence of Melafen concentration. We conclude that the three
main types of purinoreceptors: metabotropic P2Y and two Ca
2+
channel formers P2X
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