From the Producer Microorganisms to the Lasso Scaffold - Lasso Peptides: Bacterial Strategies to Make and Maintain Bioactive Entangled Scaffolds - page 9

Table 2.1 (continued)

WĞƉƟĚĞ WƋŽĚƵĐŜŶŐƐƚƋĂŜŶ ^ŽƵƋĐĞ ZĞĨĞƋĞŶĐĞƐ

WZKDKDZ/ Đ

ĂƉŜƐƚƋƵŜŶ ƵƋŬŚŽŮĚĞƋŜĂ ŶǀŜƋŽŶŴĞŶƚĂŮƐĂŴƉŮĞ͕ ;<ŶĂƉƉĞĞƚĂŮ͘ϮϬϬϴͿ

ƚŚĂŜŮĂŶĚĞŶƐŜƐ
ϮϹΚ^DϬϮϮϳϹ
;ĎĞƚĂͿ

DŚĂŜŮĂŶĚ;ƋĞƩĞƚĂŮ͘
ϬΕΕϴͿ

ĂƵŮŽƐĞŐŶŜŶ/
ĂƵŮŽƐĞŐŶŜŶ//
ĂƵŮŽƐĞŐŶŜŶ///

ĂƵŮŽĎĂĐƚĞƋƐĞŐŶŜƐ
^DϳϬϮϬ
;ĂŮƉŚĂͿ

^ŽŜŮƐĂŴƉŮĞ ;,ĞŐĞŴĂŶŶĞƚĂŮ͘ϮϬϬϮĂͿ

DŜĐƋŽĐŜŶ:ϮΡ ƐĐŚĞƋŜĐŚŜĂĐŽŮŜ ZϮΡ

;ŐĂŴŴĂͿ

&ĞĐĞƐŽĨĂŶĞǁĎŽƋŶ
ŜŶĨĂŶƚ͕ƋŐĞŶƟŶĂ

;^ĂŮŽŴSŶĂŶĚ&ĂƋŞĂƐ
ϬΕΕϮͿ

ZŚŽĚĂŶŽĚŜŶ Ě ZŚŽĚĂŶŽĎĂĐƚĞƋ

ƚŚŜŽŽDŽLJĚĂŶƐ >^Ϯ^D
ϬϴϴϹϮ
;ŐĂŴŴĂͿ

ŜŽĮŮŴŽŶ
ƐƵŮĨƵƋƉĂƋƟĐŮĞƐ;>ĞĞĞƚĂŮ͘
ϮϬϬϳͿ

;,ĞŐĞŴĂŶŶĞƚĂŮ͘ϮϬϬϮĎͿ

ZƵĎƋŜǀŜŶŽĚŜŶ ZƵĎƋŜǀŜǀĂDŽŐĞŮĂƟŶŽƐƵƐ

/>ΚΚEZϬϬϬϮΚΡ
;ĎĞƚĂͿ

&ŽŽĚǁĂƐƚĞǁĂƚĞƋŜŶ
EĂŐĂŶŽ͕:ĂƉĂŶ;,ŽƐŚŜŶŽ
ĂŶĚ^ĂƚŽŚϬΕϴΡͿ

;,ĞŐĞŴĂŶŶĞƚĂŮ͘ϮϬϬϮĎͿ

^ƉŚŜŶŐŽŶŽĚŜŶ/ Ě
^ƉŚŜŶŐŽŶŽĚŜŶ// Ě

^ƉŚŜŶŐŽĎŜƵŴŨĂƉŽŶŜĐƵŴ
HDϮϹ^DϬϹΚϬϮ
;ĂŮƉŚĂͿ

^ŽŜŮƐĂŴƉŮĞ;WĂŮĞƚĂŮ͘
ϮϬϬΡͿ

;,ĞŐĞŴĂŶŶĞƚĂŮ͘ϮϬϬϮĎͿ

^ƉŚŜŶŐŽƉLJDŽŜŶ/ Ě
^ƉŚŜŶŐŽƉLJDŽŜŶ// Ě

^ƉŚŜŶŐŽƉLJDŽŜƐĂŮĂƐŬĞŶƐŜƐ
ZϮϮΡϹ^DϬϮΡΕϮ
;ĂŮƉŚĂͿ

^ĞĂǁĂƚĞƋ͕'ƵŮĨŽĨŮĂƐŬĂ
;SĂŶĐĂŶŶĞLJƚĞƚĂŮ͘ϮϬϬϬͿ

;,ĞŐĞŴĂŶŶĞƚĂŮ͘ϮϬϬϮĎͿ

^LJĂŶŽĚŜŶ/ Ě ^ƉŚŜŶŐŽĎŜƵŴLJĂŶŽŜŬƵLJĂĞ

Y>EϮͲΡ
;ĂŮƉŚĂͿ

WĞƚƋŽŮĞƵŴͲ
ĐŽŶƚĂŴŜŶĂƚĞĚƐŽŜŮƐ;'ĂŜĞƚ
ĂŮ͘ϮϬϬϳͿ

;,ĞŐĞŴĂŶŶĞƚĂŮ͘ϮϬϬϮĎͿ

YĂŶƚŚŽŴŽŶŜŶ/ Ě
YĂŶƚŚŽŴŽŶŜŶ// Ě

YĂŶƚŚŽŴŽŶĂƐŐĂƋĚŶĞƋŜ
^DϬΕϬϮϳͬDϬΕϴϹΡ

WŮĂŶƚƉĂƚŚŽŐĞŶ;:ŽŶĞƐĞƚ
ĂŮ͘ϮϬϬΚͿ

;,ĞŐĞŴĂŶŶĞƚĂŮ͘ϮϬϬΚͿ

YĂŶƚŚŽŴŽŶŜŶ/// Ě YĂŶƚŚŽŴŽŶĂƐĐŜƚƋŜ Ɖǀ

ŴĂŶŐŜĨĞƋĂĞŜŶĚŜĐĂĞ
>D'ϮΚϬDϬϬϹϮϳ
;ŐĂŴŴĂͿ

&ƋƵŜƚƉĂƚŚŽŐĞŶ;ŽŜĚŐĞ
ϬΕϬΡͿ

ƵĐŜŶŽĚŜŶ

WŚĞŶLJŮŽĎĂĐƚĞƋŜƵŴ
NJƵĐŜŶĞƵŴ ,><Ϭ
;ĂŮƉŚĂͿ

/ŶƚƋĂĐĞŮŮƵŮĂƋĎĂĐƚĞƋŜƵŴ
ŜƐŽŮĂƚĞĚĨƋŽŴŚƵŴĂŶ
ĞƋLJƚŚƋŽŮĞƵŬĞŴŜĂĐĞŮŮ
ŮŜŶĞ;ŚĂŶŐĞƚĂŮ͘ϮϬϬϳͿ

;,ĞŐĞŴĂŶŶĞƚĂŮ͘ϮϬϬϮĎͿ

Ă͘ >ĂƋŜĂƟŶŜƐƚŚĞϬϴĂŴŜŶŽĂĐŜĚĨŽƋŴŽĨŮĂƋŜĂƟŶ;ŶŽǁƚĞƋŴĞĚŮĂƋŜĂƟŶͿ͕ƚƋƵŶĐĂƚĞĚĂƚƚŚĞͲƚĞƋŴŜŶƵƐ͘
Ď͘ Z^ͲϳϬϬͲϮĂŶĚZ^ͲϳϬϬͲΚĂƋĞŽDŽŜĚŜNJĞĚĨŽƋŴƐŽĨZ^ͲϳϬϬͲϬĂŶĚZ^ͲϳϬϬͲϮ͕ƋĞƐƉĞĐƟǀĞŮLJ͘DŚĞLJĂƋĞƚŚƵƐ
ŶŽƚĐŽŶƐŜĚĞƋĞĚŚĞƋĞĂƐŜŶĚŜǀŜĚƵĂŮŮĂƐƐŽƉĞƉƟĚĞƐ͘
Đ͘ DŚĞĐŮĂƐƐŽĨƚŚĞƉƋŽƚĞŽĎĂĐƚĞƋŜĂŮƐƚƋĂŜŶƐŜƐŜŶĚŜĐĂƚĞĚŜŶĎƋĂĐŬĞƚƐĂŌĞƋƚŚĞŶĂŴĞŽĨƚŚĞƐƚƋĂŜŶ͘
Ě͘ DŚĞŮĂƐƐŽƉĞƉƟĚĞƐŜƐŽŮĂƚĞĚĐŽƋƋĞƐƉŽŶĚƚŽƚƋƵŶĐĂƚĞĚĨŽƋŴƐŽĨƚŚĞƉĞƉƟĚĞƐƉƋĞĚŜĐƚĞĚĨƋŽŴƚŚĞ
ƉƋĞĐƵƋƐŽƋŐĞŶĞƐĞƋƵĞŶĐĞ͘

represent a prototype of lasso gene clusters. Therefore, the nomenclature ABCD is
recommended by the RiPPs community to describe these clusters (Arnison et al.
2013 ). The first gene cluster from Actinobacteria was characterized for lariatins
in Rhodococcus jostii K01-B0171 (Inokoshi et al. 2012 ), in which the B-like pro-
tein was split and encoded by two genes. The genome-wide survey that followed,
revealed widespread distribution of mcj / cap -like clusters in microbial genomes as
well as deviations in the gene organization (Severinov et al. 2007 ; Maksimov et al.
2012 ; Hegemann et al. 2013b ). Currently 25 out of 35 known lasso peptides have