Analyzing the Biological Entity of Nanomaterials - Safety of Nanomaterials along Their Lifecycle - page 57

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Analyzing the Biological
Entity of Nanomaterials
Characterization of
Nanomaterial Properties
in Biological Matrices

Christian A. Ruge, Marc D. Driessen,
Andrea Haase, Ulrich F. Schäfer,
Andreas Luch, and Claus-Michael Lehr

CONTENTS
4.1 Introduction .................................................................................................... 60
4.1.1

Why Is It Important to Characterize Nanomaterials In Situ ? ............. 60

4.1.2 Interaction of Nanoparticles with Proteins and Influencing Factors .... 63

4.1.3 Methodological Approaches for In Situ Characterization of

Nanomaterials ..................................................................................... 65

4.1.4 Biological Fluids as Model Systems to Study Bio-Nano

Interactions ....................................................................................... 68

4.2 Nanomaterial Interactions with Serum or Serum-Containing Cell

Culture Medium.............................................................................................. 71
4.2.1

Physiological Considerations .............................................................. 71

4.2.2 Available Models and Lessons Learned from Published Studies ...... 72

4.2.3 Single Protein Systems ....................................................................... 73

4.2.4 Interaction with Plasma or Serum ...................................................... 74

4.2.5 Lessons Learned from NanoGEM ..................................................... 75

4.2.6 Conclusions and Considerations for Future Studies ........................... 77

4.3 Example/Case Study: Nanomaterial Interactions with Biological Fluids

in the Respiratory Tract .................................................................................. 78
4.3.1 Physiological Considerations .............................................................. 78

4.3.2 Available Models for Lung Lining Fluid ............................................ 79

4.3.3 Lessons Learned from Published Studies .......................................... 81

4.3.4 Lessons Learned from NanoGEM ..................................................... 83

4.3.5 Conclusions and Considerations for Future Studies ........................... 85

4.4 Example/Case Study: Nanomaterial Interactions with Biological Fluids

in the Gastrointestinal Tract ........................................................................... 85

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