Measurement of Nanoparticle Uptake by Alveolar Macrophages - Safety of Nanomaterials along Their Lifecycle - page 135

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Measurement of
Nanoparticle Uptake by
Alveolar Macrophages
A New Approach Based on
Quantitative Image Analysis

Darius Schippritt, Hans-Gerd Lipinski,
and Martin Wiemann

CONTENTS
7.1 Introduction .................................................................................................. 138
7.2 Materials and Methods ................................................................................. 139
7.2.1 Cells .................................................................................................. 139
7.2.2 Setting up Observation Experiments ................................................ 139
7.2.3 Particle Suspensions ......................................................................... 140
7.2.4 Scanning Electron Microscopy ........................................................ 140
7.2.5 Particle Tracking Analysis ................................................................ 141
7.2.6 General Requirements for the Software Development ..................... 141
7.3 Results ........................................................................................................... 141
7.3.1 Sedimentation of Agglomerated Nanoparticles in Cell
Culture Medium................................................................................ 141
7.3.1.1 Quantity of Sedimented Agglomerates .............................. 141
7.3.1.2 Conversion of Measured Particle Size into
Particle Mass .................................................................. 14 4
7.3.1.3 Gravitational Settling of ZrO 2 Nanoparticles .................... 145
7.3.2 Phagocytosis of Agglomerated Nanoparticles .................................. 145
7.3.2.1 Detection and Tracking of Macrophages ........................... 146
7.3.2.2 Measuring the Uptake of Agglomerated Particles ............. 148
7.3.2.3 Model-Based Description of Phagocytosis ........................ 150
7.3.2.4 Experimental Data of Particle Uptake ............................... 152
7.4 Discussion and Perspectives ......................................................................... 154
Acknowledgments .................................................................................................. 156
References .............................................................................................................. 157

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