Analyzing the Biological Entity of Nanomaterials - Safety of Nanomaterials along Their Lifecycle - page 88

GLOSSARY
Ex situ

Analysis of nanomaterials after prior re-isolation of the nanomaterial out of a test matrix
(i.e., after incubation with biomolecules, the nanomaterials are isolated to separate
from non-bound materials to perform the analysis of adsorbed nanomaterials).

Ex vivo Experiments performed with whole isolated organs.

In situ Analysis of nanomaterials inside a test matrix (e.g., inside cells, tissues, or in

biologically relevant test fluids) without any processing or re-isolation of the
nanomaterial before analysis.

In vitro Experiments performed under simplified conditions using isolated components of the

organism (cell lines or primary cells in single culture or co-culture).

In vivo Experiments performed using whole organisms (i.e., with maximal biological

complexity).

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