Biology Reference
In-Depth Information
undoubtedly lead to new applications, including sequential photoactivation
schemes that can be advantageous for unraveling complex protein-protein interac-
tions. Optimization of conversion efficiencies, thermal rates, and dynamic ranges
are still important tasks to be performed on these proteins. While this research is
strongly motivated by applications in cell biology, it will also yield new basic
insights into the physical chemistry of chromophore-solvent
interactions and
protein dynamics.
Acknowledgments G.U.N. was supported by the Deutsche Forschungsgemeinschaft (DFG) and
the State of Baden-Wurttemberg through the Center for Functional Nanostructures (CFN), by DFG
grant Ni 291/9 and by the Baden-Wurttemberg Stiftung. J.W. acknowledges funding by DFG grant
Wi 1990/2, the Network Fluorescence Applications in Biotechnology and Life Sciences, FABLS,
Australia, the Landesstiftung Baden-Wurttemberg (Elite Postdoc Program); the Natural Environ-
ment Research Council, UK (NE/G009643/1) and the University of Southampton.
References
1. Nienhaus GU (2008) The green fluorescent protein: a key tool to study chemical processes in
living cells. Angew Chem Int Ed Engl 47:8992-8994
2. Shimomura O, Johnson FH, Saiga Y (1962) Extraction, purification and properties of
aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J Cell
Comp Physiol 59:223-239
3. Chalfie M, Tu Y, Euskirchen G et al (1994) Green fluorescent protein as a marker for gene
expression. Science 263:802-805
4. Prasher DC, Eckenrode VK, Ward WW et al (1992) Primary structure of the Aequorea
victoria green-fluorescent protein. Gene 111:229-233
5. Tsien RY (1998) The green fluorescent protein. Annu Rev Biochem 67:509-544
6. Matz MV, Fradkov AF, Labas YA et al (1999) Fluorescent proteins from nonbioluminescent
Anthozoa species. Nat Biotechnol 17:969-973
7. Wiedenmann J, Elke C, Spindler KD et al (2000) Cracks in the beta -can: fluorescent proteins
from Anemonia sulcata (Anthozoa, Actinaria). Proc Natl Acad Sci USA 97:14091-14096
8. Wiedenmann J (1997) Die Anwendung eines fluoreszierenden Proteins und weiterer farbiger
Proteine und der zugehorigen Gene aus der Artengruppe Anemonia sp. (sulcata) Pennant,
(Cnidaria, Anthozoa, Actinaria) in Gentechnologie und Molekularbiologie. Offenlegungss-
chrift DE 197 18 640 A1, Deutsches Patent- und Markenamt, pp 1-18. In, Offenlegungsschrift
DE 197 18 640 A1: Deutsches Patent- und Markenamt; 1997:1-18
9. Wiedenmann J, Nienhaus GU (2006) Live-cell imaging with EosFP and other photoactiva-
table marker proteins of the GFP family. Expert Rev Proteomics 3:361-374
10. Patterson GH, Lippincott-Schwartz J (2002) A photoactivatable GFP for selective photolabel-
ing of proteins and cells. Science 297:1873-1877
11. Habuchi S, Ando R, Dedecker P et al (2005) Reversible single-molecule photoswitching in the
GFP-like fluorescent protein Dronpa. Proc Natl Acad Sci U S A 102:9511-9516
12. Adam V, Lelimousin M, Boehme S et al (2008) Structural characterization of IrisFP, an
optical highlighter undergoing multiple photo-induced transformations. Proc Natl Acad Sci U
S A 105:18343-18348
13. Ormo M, Cubitt AB, Kallio K et al (1996) Crystal structure of the Aequorea victoria green
fluorescent protein. Science 273:1392-1395
14. Yang F, Moss LG, Phillips GN Jr (1996) The molecular structure of green fluorescent protein.
Nat Biotechnol 14:1246-1251
Search WWH ::
Custom Search