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undoubtedly lead to new applications, including sequential photoactivation
schemes that can be advantageous for unraveling complex protein-protein interac-
tions. Optimization of conversion efficiencies, thermal rates, and dynamic ranges
are still important tasks to be performed on these proteins. While this research is
strongly motivated by applications in cell biology, it will also yield new basic
insights into the physical chemistry of chromophore-solvent
interactions and
protein dynamics.
Acknowledgments G.U.N. was supported by the Deutsche Forschungsgemeinschaft (DFG) and
the State of Baden-Wurttemberg through the Center for Functional Nanostructures (CFN), by DFG
grant Ni 291/9 and by the Baden-Wurttemberg Stiftung. J.W. acknowledges funding by DFG grant
Wi 1990/2, the Network Fluorescence Applications in Biotechnology and Life Sciences, FABLS,
Australia, the Landesstiftung Baden-Wurttemberg (Elite Postdoc Program); the Natural Environ-
ment Research Council, UK (NE/G009643/1) and the University of Southampton.
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