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Fig. 8 Pulse-chase imaging with super-resolution PALM using fusion proteins with mIrisFP.
(a)-(d) Schematic depiction of the experiment. (a) Only a few protein molecules fluoresce in the
green at any given point in time (
bright green dots
); the majority is in the dark state (
dark-green
dots
). By sequential activation and localization, a PALM image can be taken. (b) Molecules in the
area marked by the
violet rectangle
are photoconverted to the red form by 400-nm light irradiation.
(c) Again, only a small subfraction is in the fluorescent state at any point in time (
bright red dots
),
the majority is in the dark state (
dark-red dots
). (d)
Red molecules
migrate to other regions of the
cell that were not irradiated with 400-nm light, where they are subsequently localized. (e) PALM
image using
green IrisFP
.(f) Targeted photoconversion of a mIrisFP subpopulation to the
red
state
.(g) PALM image using
red mIrisFP
, showing that
red-converted molecules
migrate to other
regions of the cell
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