Biology Reference
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Fig. 10 Histograms of
emission peak positions for
DsRed and variants. The
emission from the red-
emitting chromophore is
systematically red-shifted for
all variants compared to the
bulk emission, and is
attributed to the rapid
formation of a super-red form
of the chromophore. The
emission maximum position
from the green-emitting
chromophore agrees with the
ensemble peak of this
chromophore for all variants.
The histogram further shows
the emission maximum
positions of rare forms
emitting in the wavelength
area between the typical
emission of the green- and the
red-emitting chromophore
(see inset in the
top panel
)
(From [
88
], Copyright Wiley-
VCH Verlag GmbH & Co.
KGaG. Reproduced with
permission)
green-emitting
chromophore
rare
forms
red-emitting
chromophore
600
DsRed
ensemble
maximum
500
10
400
300
5
200
0
520
540
560
580
100
λ
/nm
0
DsRed2
300
200
ensemble
maximum
100
0
Fluorescent
Timer
150
100
ensemble
maximum
50
0
ensemble
maxima
DsRed
N42H
500
400
300
200
100
0
AG4
ensemble
maximum
200
100
0
500 520 540 560 580 600 620
λ
/nm
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