One-Photon and Two-Photon Excitation of Fluorescent Proteins - Fluorescent Proteins: From Understanding to Design - page 8

Table 1 Optical properties of representative fluorescent proteins
Name a

QY c

Original
pr otein

Chromopho
re b

Exc/Abs
peak
(nm)

Em peak
(nm)

Ext.
coeff
(10 3 M -1
cm -1 )

References

GFP Y66F av GFP BFPF 360 442 (low)

-

[11]
[20]
[21]

EBFP av GFP BFP 377 446 30 0.15

EBFP2 av GFP BFP 383 448 32 0.56

av GFP BFP 384 450 26.2 0.55

Azurite

[22]

mKalama1 av GFP GFPn 385 456 36 0.45

[21]
[21]
[23]
[115]

mBlueberry2 DsRed RFP Y67F 402 467 51 0.48

Cerulean av GFP CFP 433 475 43 0.62

ECFP av GFP CFP 434 476 32.5 0.4

424/440 d 461/482 d mTFP1-Y67W amFP486 CFP 13 0.02 [24]

cFP484 (tetr) - GFPa 456 484 35.3 0.48

[6]
[6]

amFP486 (tetr) - GFPa 458 486 40 0.24

mTFP1 amFP486 GFPa 462 492 64 0.85 [24]

[20]

EGFP av GFP GFPa 484 507 53 0.6

GFP R - state e av

- GFPa 477 508 9.5 0.72

[116]
[116]

510 (ESPT) f

av GFP RHstate - GFPn 397 25 0.78

T-Sapphire av GFP GFPn 399 511 (ESPT) 44 0.60

[25]
[26]
[27]
[28]
[29]
[30]

22G GFPa 503 518 95 0.85

Dronpa

EYFP av GFP GFPa 514 527 84 0.61

Venus av GFP GFPa 515 528 92.2 0.57

YPet av GFP GFPa 517 530 104 0.77

PhiYFP (dim) - GFPa 525 537 115 0.60

- zFP538 528 538 - 0.42

zFP538 (dim) [6]

mKO KO mKO 548 561 51.6 0.60 [31]

[32]
[32]

487/504 d 537/562 d

mHoneydew DsRed RFP Y67W 17 0.12

mOrange DsRed mOrange 548 562 71 0.69

Dendra pa g DendFP uvKaede 557 575 20 0.72 [33]

[34]
[35]

EosFP pa (tetr) - uvKaede 571 581 41 0.55

Kaede pa (tetr) - uvKaede 572 582 60.4 0.33

DsRed (tetr) - RFP 558 583 57 0.79

[6]

asFP595 pa (tetr) - asFP595 568 595 56.2 <10 -3 [36]

mStrawberry DsRed RFP 574 596 90 0.29 [32]

KFP1 pa (tetr) AsFP595 asFP595 580 600 59 0.07

[117]

mRFP1 DsRed RFP 584 607 44 0.25 [37]

mCherry DsRed RFP 587 610 72 0.22

[32]

- trans RFP 559 611 78 0.45

eqFP611 (tetr)

[38]

<10 -3 Rtms5 (tetr) - trans RFP 592 620 80

[39]
[40]
[41]
[42]

mPlum DsRed RFP 590 649 41 0.10

mNeptune eqFP578 RFP 600 650 67 0.20

cjBlue - RFP 610 - 66.7 -

a

The oligomerization state, i.e., dimer (dim) or tetramer (tetr), is reported for nonmonomeric
proteins
b Chromophore name refers to Fig. 4
c QY

Quantum yield
d Two excitation/fluorescent peaks are present
e RH state and R state for av GFP refer to the two bands assigned to neutral (GFPn) and anionic
(GFPa) protonation state of the chromophore
f ESPT in the fourth column indicates that emission from the anionic species takes place after
excited state proton transfer
g pa

¼

¼ after photoactivation