Vibrational Spectroscopy of Fluorescent Proteins: A Tool to Investigate the Structure of the Chromophore and Its Environment - Fluorescent Proteins: From Understanding to Design - page 160

Fig. 8 (a) Blue lines : femtosecond-stimulated Raman scattering (FSRS) spectra of wt GFP in D 2 O,
from 100 fs to 1 ps following 396-nm excitation; Gaussian fits are shown as dashed red lines on

each spectrum. (b) Time dependence of vibrational structural features frequencies; they oscillate
with a period of ~280 fs. The C N stretching, imidazolinone-ring deformation, and (C)O-D

rocking modes are in phase, and this group of modes oscillates out of phase with the C-O

¼