Vibrational Spectroscopy of Fluorescent Proteins: A Tool to Investigate the Structure of the Chromophore and Its Environment - Fluorescent Proteins: From Understanding to Design - page 147

Table 1 (continued)
Mode frequency (cm 1 )
chromophore - protein

Mode description Activity Measurement in isolated

chromophore

Measurement
in protein

Assignment (theory)

1,009 IR, R [ 35 ]

930 IR, R, RR, SERS [ 9 , 13 , 35 ] [ 13 ]

910 - 910 IR, SERS [ 13 , 35 ] [ 13 ] [ 13 ]

850 - 875 Rings breathing IR, R, RR, SERS [ 9 , 13 , 35 ] [ 13 ] [ 13 ]

840 IR [ 35 ]

823 IR [ 35 ]

805 - 830 R, RR, SERS [ 9 , 13 , 35 ] [ 13 ] [ 13 ]

781 IR, SERS [ 13 , 35 ] [ 13 ] [ 13 ]

750 IR, R, RR, SERS [ 9 , 13 , 35 ] [ 13 ]

715 - 720 Pl rings + bridge IR, R, RR, SERS [ 4 , 9 , 13 , 35 ] [ 4 , 13 ] [ 4 , 13 ]

657 - 650 IR, SERS [ 13 , 35 ] [ 13 ] [ 13 ]

636 IR [ 35 ]

606 Pl imid + methil IR, R, RR, SERS [ 4 , 9 , 13 , 35 ] [ 13 ] [ 4 ]

580 IR, R, RR [ 9 , 35 ]

536 IR [ 35 ]

520 Phenol + OH SERS [ 13 ] [ 13 ]

512 IR, R, RR [ 9 , 35 ]

489 - 500 Phenol + OH SERS [ 13 ] [ 13 ] [ 13 ]

415 - 400 Phenol + OH SERS [ 13 ] [ 13 ] [ 13 ]

364 SERS [ 13 ] [ 13 ] [ 13 ]

(b) Anionic chromophore
1,665 - ~ 1,663 C ¼ O stretch IR [ 9 , 37 ] [ 9 , 37 - 39 ]

1,630 - ~ 1 , 620 C ¼ C stretch IR, RR [ 9 , 36 , 37 ] [ 36 ] [ 37 - 39 ]

1,580 - 1 , 603 Phenol (+C-O) IR, RR [ 9 , 36 , 37 ] [ 36 ] [ 37 ]

1,560 - 1 , 565 C ¼ N (+C ¼ C) IR, RR [ 9 , 36 , 37 ] [ 36 ] [ 37 , 39 ]

1,530 Phenol RR [ 9 , 36 , 37 ] [ 36 ] [ 37 ]