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Fig. 3.7 Phylogenetic diversity of the Sargasso Sea se-
quences computed by MEGAN. The microheterogeneity
of sample 1 was investigated by comparing it to pooled
samples 2, 3, and 4 (Venter et al. 2004 ). a Analysis of
10,000 reads randomly chosen from Sample 1. b Analy-
sis of 10,000 reads randomly chosen from Sample 2. c,
d A more detailed view of sample 1 and samples 2-4,
respectively, illustrating a significant difference of rela-
tive frequencies of Shewanella and Burkholderia spe-
cies in the two data sets. In all such Figs. (Fig. 3.7 ), each
circle represents a taxon in the NCBI taxonomy and is
labeled by its name and the number of reads that are as-
signed either directly to the taxon, or indirectly via one of
its subtaxa. The size of the circle is scaled logarithmically
to represent the number of reads assigned directly to the
taxon. (Huson et al. 2007 )
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