Management of Environmental Phosphorus Pollution Using Phytases: Current Challenges and Future Prospects - Applied Environmental Biotechnology: Present Scenario and Future Trends - page 108

production of phytase to mineralise phytate, will
have potential to be used in soil with high content
of organic P.
Considering the facts and problems, an inte-
grated approach from a combined agricultural
and environmental perspective is recommended
in order to achieve better P management with
reduced environmental pollution. The follow-
ing four recommendations would contribute to
a more equilibrated use of the available P re-
sources. First , consistent and regular quality
analysis of soils and fertilisers for optimum P
concentration is required for determining actual
P concentration and the dose of fertiliser to be ap-
plied. Possible changes in management practice
such as better soil conservation; better precision
in applications of fertiliser and extensification of
agricultural systems such as livestock reductions
may be useful in tackling the problem. Second ,
because many farmers are not familiar with the
use of manure products, its proper use, disposal
and related P content, the methodology and ad-
vantages in the use of phytases as a new approach
should be made known to them through aware-
ness campaigns. Third , cost monitoring of phy-
tase-supplemented animal feed is also required
for its maximum utilisation and reach to farmers.
Fourth , development of biofertilisers that aid in
better P solubility and its availability to plants
will certainly be useful in the long term control
of P pollution.

Augspurger NR, Webel DM, Lei XG, Baker DH (2003)
Efficacy of an E. coli phytase expressed in yeast for
releasing phytate-bound phosphorus in young chicks
and pigs. J Anim Sci 81(2):474-483
Barrientos L, Scott JJ, Murthy PP (1994) Specificity of
hydrolysis of phytic acid by alkaline phytase from lily
pollen. Plant Physiol 106:1489-1495
Bertrand JA, Flech JC, McConnell JCJ (1999) Phos-
phorus intake and excretion on South Carolina dairy
farms. Prof Anim Sci 15:264-267
Black CA (1968) Soil-plant relationships. Wiley, New
York
Bogar B, Szakacs G, Pandey A, Abdulhameed S, Linden
JC, Tengerdy RP (2003) Production of phytase by
Mucor racemosus in solid-state fermentation. Bio-
technol Prog 19:312-319
Bohn L, Meyer A, Rasmussen S (2008) Phytate: impact
on environment and human nutrition. A challenge for
molecular breeding. J. Zhejiang Univ Sci B 9:165-191
Bomans E, Fransen K, Gobin A, Mertens J, Michiels P,
Vandendriessche H, Vogels N (2005) Addressing phos-
phorus related problems in farm practice. Final report to
the European Commission, DG Environment, pp 9-21
Boyce A, Walsh, G (2007) Purification and characterisa-
tion of an acid phosphatase with phytase activity from
Mucor hiemalis Wehmer, J Biotechnol 132:82-87
Cao L, Wang W, Yang C, Yang Y, Diana J, Yakupitiyage
A, Luo Z, Li D (2007) Application of microbial phy-
tase in fish feed. Enzyme Microb Technol 40:497-507
Casey A, Walsh G (2004) Identification and characteriza-
tion of a phytase of potential commercial interest. J
Biotechnol 110:313-322
Centner T (2004) Developing institutions to encourage
the use of animal wastes as production inputs. Agric
Hum Values 21:367-375
Chadha BS, Gulati H, Minhas M, Saini HS, Singh N
(2004) Phytase production by the thermophilic fungus
Rhizomucor pusillus , World J Microbiol Biotechnol
20:105-109
Cho JS, Lee CW, Kang SH, Lee JC, Bok JD, Moon YS,
Lee HG, Kim SC, Choi YJ (2003) Purification and
characterization of a phytase from Pseudomonas
syringae MOK1. Curr Microbiol 47:290-294
Cowieson A, Acamovic T, Bedford M (2006) Phytic acid
and phytase: implications for protein utilization by
poultry. Poult Sci 85:878-885
Dobrota C (2004) The biology of phosphorous. In Val-
sami-Jones, E. (ed) Phosphorus in environmental tech-
nologies. IWA, London, pp 51-74
Dost K, Tokul O (2006) Determination of phytic acid in
wheat and wheat products by reverse phase high per-
formance liquid chromatography. Anal Chim Acta
558:22-27
Dvor￡kov￡ J (1998) Phytase: sources, preparation and
exploitation. Folia Microbiol 43:323-338
Ebune AS, Al-Asheh, Duvnjak Z (1995) Effects of phos-
phate, surfactants and glucose on phytase production
and hydrolysis of phytic acid in canola meal by Asper-
gillus ficuum during solid-state fermentation. Biore-
source technology 54:241-247

References

Abioye S, Ige D, Akinremi O, Hu Y, Flaten DN (2010)
Characterizing fecal and manure phosphorus from
pigs fed phytase supplemented diets. J Agr Sci
2:1916-9752.
Aehle W (2007) Industrial enzymes. In Aehle W (ed)
Enzymes in industry: production and applications, 3rd
edn. Wiley-VCH, Weinheim, pp 99-263
Angel CR, Powers WJ, Applegate TD, Tamim NM,
Christma MC (2005) Influence of phytase on water-
soluble phosphorus in poultry and swine manure. J
Environ Qual 34:563-571
Angelis MD, Gallo G, Corbo MR, McSweeney PLH, Fac-
cia M, Giovine M, Gobbetti M (2003) Phytase activ-
ity in sourdough lactic acid bacteria: puri fi cation and
characterization of a phytase from Lactobacillus san-
franciscensis CB1. Int J Food Microbiol 87:259-270