Water Use and Drought Response in Cultivated and Wild Apples - Abiotic Stress: Plant Responses and Applications in Agriculture - page 253

Genes Up-regulated in
'Royal Gala' Roots

Other Plants Tissue Citation

Arabidopsis
Poplar proteome

various
white roots

[40] 2
[41]

HMW HSP

Arabidopsis
barley
maize
Poplar proteome

various
leaves and roots
leaves and roots
white roots

[40]
[35]
[43]
[41]

aquaporin

Arabidopsis
chickpea

various
whole seedlings

protease inhibitor [40,42]

Arabidopsis
chickpea
maize

various
whole seedlings
leaves and roots

[40]
[42]
[43]

Histone H2

1 Apple ('Royal Gala') genes are from two SSH root libraries of water deficit-treated plants (manuscript submitted)

2 This citation is a review of several different studies of water deficit-response in Arabidopsis .

Table 1. Genes up-regulated in water deficit-treated apple roots vs other plants responding to water deficit 1

density between lines. Interestingly, there was no compensation in biomass for the reduction
in E, indicating that as with WUE and ∆ 13 C, there are workable strategies for increasing WUE
without sacrificing carbon assimilation under normal growth conditions.

Using a suppression subtractive hybridization (SSH) approach to drought-responsive gene
isolation in a commercial apple, 'Royal Gala', we identified numerous genes commonly found
to be WD responsive in other plants. We also identified several up-regulated genes unique to
apple roots (Table 2; manuscript submitted). Some of these genes may reflect the role of roots
in nutrient transport during stress, including a copper chaperone and a high affinity nitrate
transporter that is a putative Arabidopsis NRT2.4 homolog.

Genes Up-regulated in 'Royal Gala'

Roots Bark Leaves

BYPASS1 Anthocyanin reductase Auxin/Aluminum-induced protein

Serine acetyltransferase GAST1-like gene Proteasome maturation factor

High affinity nitrate transporter Asparagine synthetase Asparagine synthetase

NPR1 Chloroplast membrane protein Tic40 Glyoxylate aminotransferase

1 These genes have not yet been reported as up-regulated in response to WD in these tissues of other plants.

Table 2. Genes uniquely up-regulated in WD-treated apple tissues 1