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The second step of OH formation is the Fenton reaction in which reduced form of iron
(Fe 2+ ) transfers electrons to H 2 O 2 :
Fe 2+ + H 2 O 2 → Fe 3+ + OH + OH
O 2 •− can be also protonated to form HO 2 . Furthermore, O 2 •− can react with another free radi‐
cal species as NO to generate peroxynitrite (OONO ) [66]. Another form of ROS - singlet
oxygen is the first excited electron state of O 2 that originates when an electron is elevated to
a higher energy orbital (Figure 3).
Figure 2. ROS production and programmed cell death induced by abiotic stress (according to Gill and Tuteja,
2010) [66].
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