Biology Reference
In-Depth Information
Figure 5.1. The cheek hair pattern variation found on the two subspecies of
Macaca fascicularis
living in Thailand. a)
Macaca fascicularis fascicularis
with a
transzygomatic pattern of cheek hair living at Wat Thammasala, Nakhon Pathom
Province (13° 48' 42”N, 100° 06' 52”E) and b)
M. f. aurea
with an infrazygomatic
pattern of cheek hair living at Wat Paknam Pracharangsarith, Ranong Province
(9° 57' 11”N, 98° 35' 41”E) (photographs by S. Malaivijitnond).
878 long-tailed macaques were caught between 2003 and 2007. All of them
were wild or semi-wild macaques inhabiting the hills near temples (or Wat in
Thai), recreation parks, or mangrove forests. Monkeys were anesthetized by
intramuscular injection with 10mg/kg body weight of ketamine hydrochloride.
While the monkeys were immobilized, body mass, tail length, crown-rump
length, and pelage color were recorded (Hamada
et al
.,
2006
;
2008
), blood was
collected by femoral venipuncture (Malaivijitnond
et al
.,
2007b
), and feces
were collected directly from the anus (Malaivijitnond
et al
.,
2006
). Blood sam-
ples were preserved in heparin or EDTA buffer, and fecal samples were mixed
vigorously and homogenously with 2-4 ml of 10 percent formalin buffer. The
DNA was extracted from the white blood cells and kept as a DNA bank at the
Primate Research Unit of Chulalongkorn University. The monkeys were sub-
jected to various clinical inspections including external parasites before being
released back into the troop after their complete recovery from anesthesia. The
ages of monkeys were estimated from the dental eruption according to Smith
et al
., (
1994
).
